Cell pairings for ascidian embryo registration
Michelin G., Guignard L., Fiuza U.-M., Lemaire P., Godin C., Malandain G.
15 avr. 2015
Recent microscopy techniques allow imaging temporal 3D stacks of developing organs or embryos with a cellular level of resolution and with a sufficient acquisition frequency to accurately track cell lineages. Imaging multiple organs or embryos in different experimental conditions may help decipher the impact of genetic backgrounds and environmental inputs on the developmental program. For this, we need to precisely compare distinct individuals and to compute population statistics. The first step of this procedure is to develop methods to register individuals. From a previous work of cell segmentation from microscopy images, we here demonstrate how to extract the symmetry plane of embryos at early stages, and how to use this information as a geometrical constraint to both register these embryos and obtain a cell-to-cell mapping.